Automated Single Cell Analysis Using Ultrafast Microscopy

Many of our projects make use of a range of ultrafast fluorescence microscopes we set up in the lab that can monitor multiple fluorescent reporters at over 500 locations in 96-well plates over several days. These microscopes can be integrated in experiments with automated multiplexed immunostaining for an in-depth analysis of signaling processes in tens of thousands of individual cells after live-cell tracking. Organoids are imaged using two automated spinning disk microscopes, one being setup with super-resolution imaging, and we have also two microscopes setup for automated local optogenetic manipulations of signaling activities. We also have a specialized microscopes to generate micropatterns in 96-well plates to control where cells can grow and move and one based on TIRF illumination is used to track signaling processes in the plasma membrane.

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